ANTI-DIABETIC ACTIVITIES OF ISOLATED COMPOUND BETA-SITOSTEROL FROM THE ETHANOLIC EXTRACT OF STEM OF ANDROGRAPHIS ECHIOIDESAbstract
To study the anti-oxidant and anti-diabetic activities of isolated compound beta-sitosterol from the ethanolic extract of stem of Andrographis echioides under the in-vitro model. The isolation was done using column chromatography using gradient elution with different mobile phases. Structural elucidation was carried out on the basis of spectral analysis. Anti-oxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl scavenging assay. The anti-diabetic activity was evaluated by the inhibitory potential of isolated compound beta-sitosterol against alpha-amylase and alpha-glucosidase assays. The study revealed that the beta-sitosterol exhibited significant α-amylase (58.57±0.29) and α-glucosidase (43.85±0.032) inhibitory activities respectively and well compared with standard acarbose drug. The beta-sitosterol showed the best scavenging activity (74.46±0.036) against the tested radicals like 1,1-diphenyl-2-picrylhydrazyl, The infra-red spectrum specific absorption bands for beta-sitosterol viz: 3427.78 cm‐1 (O‐H stretching.); 2937.40 cm‐1 (aliphatic C‐Hstretching); 1640.58 cm‐1 (C=C absorption peak); other absorption peaks includes 1464.31cm‐1 (CH2); 1381.56 cm‐1 (OH def), 1054.11 cm‐1 (cycloalkane) and 800.97 cm‐1. Structural elucidation of beta-sitosterol was done by spectrum analysis such as 13C and 1H depth nuclear magnetic resources. Therefore, it is concluded that beta-sitosterol is a potential source for natural anti-oxidant and anti-diabetic compounds and could have potential use in the management of diabetes mellitus.
G. Manigandan * and V. Subramaniyan
PG and Research Department of Biotechnology, J.J. College of Arts and Science, J.J Nagar, Sivapuram Post, Pudukkottai, Tamil Nadu, India.
28 August 2020
29 January 2021
28 August 2021
01 September 2021