ANTI-DIABETIC ACTIVITY OF THE METHANOLIC EXTRACT OF SEEDS OF SYZYGIUM CUMINI IN STZ-INDUCED TYPE I DIABETES IN RATSAbstract
Background: Syzygium cumini (SC) usually acknowledged as Jamun. All components of the Jamun may keep used medicinally because of a wide range of diseases like cough, diabetes, dysentery and inflammation. Objectives: Present investigational on the anti-diabetic activity of the methanolic extract seeds of SC in STZ-induced Type I Diabetes in rats. Methods: Extracted SC was to be evaluated the toxicity as OECD 425 guidelines, and biochemical, hematological then gross pathological evaluation has been assessed. Type I diabetes has been triggered in Wistar rats via STZ 65mg/kg/b.w. I.P. During the experiment, Rat’s body weight and blood glucose level were monitored. At the end of the study, animals amongst complete groups were sacrificed, and biochemical parameters; Lipid profile, C-Peptide, HbA1c, Serum insulin, pancreatic insulin, and histology on the pancreas were performed. SC was also screened for pro-inflammatory cytokines viz., IL-1β, IL-6 and TNF-α had been performed via ELISA. Furthermore, Antioxidant Enzymes as SOD, CAT, LPO, and GSH were also performed. Results: The observed extract SC was proven safe among the toxicity data. Conclusion and has been shown massive an impact on in-vivo in imitation of managing diabetic markers like weight gain, blood glucose, lipid profile, C-Peptide, HbA1c, the release of insulin secretion, and pancreatic insulin. Diabetic pancreas on rats confirmed fall of beta-cell density and disruption of normal architecture. But the treated group was observed to restore the mass of beta cells. Mediator over inflammatory cytokines kind of elevated in STZ group and was once inhibited by using SC. Elevated oxidative enzymes have also hold been seen to control the therapy with SC. Conclusion: All the findings and phytoconstituents present in the extract could keep the possible chemical compounds involved in the counteraction of diabetes.
Yousef Ahmed Saleh Haimed *, Pankaj Kumar Sharma and Deepak Kumar Jha
Department of Pharmacy, Apex University, Jaipur, Rajasthan, India.
27 December 2021
16 February 2022
30 April 2022
01 August 2022