ANTI-DIABETIC AND OXIDATIVE STRESS MARKERS IN MEN WITH TYPE 2 DIABETES MELLITUSAbstract
The aim of this study is to evaluate metformin and insulin effects on metabolic disorders and oxydatif stress markers, in Algerian men with T2D, in order to recommend the best treatment, which can minimize diabetes complications and recommend/or not a combination between metformin and insulin. Patients and Methods: We made this study on 120 subjects men divided into four groups (30 healthy control, 30 T2D without treatment, 30 T2D with metformin, and 30 T2D with insulin). Blood samples are collected for the determination of biochemical parameters (glucose, triglycerides, and cholesterol, high and low-density lipoprotein cholesterol) and oxidative markers (superoxide anion, nitric oxide, malondialdehyde, carbonyl proteins, oxygen radical absorbance capacity, vitamin C, catalase, glutathione). Results: Compared with healthy subjects, diabetic patients had altered lipid levels (cholesterol, triglycerides, LDL cholesterol) and high levels of pro-oxidant intra-cellular markers (O2•-, CP and MDA) associated to low concentrations of anti-oxidants (Vitamin C, catalase, and GSH). Our results show that insulin reduces more lipid parameters than metformin, moreover, the oxidant/antioxidant status became normal in patients treated with metformin Conclusion: Insulin treatment is more efficient than metformin treatment in improving the lipid profile. In addition, metformin, which reversed redox changes associated with diabetes and insulin, which improve all lipid profiles, should be prescribed in combination, especially in type 2 diabetes patients with hypertriglyceridemia and with severe oxidative stress.
Hasnia Rabehi, Baya Guermouche *, Hafida Merzouk, Majda Dali-Sahi and Sid Ahmed Merzouk
Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University Abou-BekrBelkaïd University, Algeria.
11 February 2021
12 May 2021
27 May 2021
01 January 2022