ANTI-INFLAMMATORY AND ANTIBACTERIAL STUDIES ON INDIGOFERA LINIFOLIA RETZ.Abstract
Indigofera linifolia Retz(Fabaceae) is herb which spread throughout the India and found to be used by the tribal to cure inflammations. Based upon the review the present study focused on screening of Ant-inflammatory and Anti-bacterial activity of Indigofera linifolia Retz. The coarse powder of whole plant was extracted by cold maceration method using various solvents successively. Preliminary phytochemical screening was carried out to confirm the presence of phytoconstituents. Based upon the phytochemical tests chloroform and ethanol extracts (200mg/kg) were taken for anti-inflammatory and antibacterial activity screening. Anti inflammatory studies was carried out by Carrageenan induced paw edema method in Wistar albino rats. Anti bacterial screening was carried out in chloroform and ethanolic extract by disc diffusion method in three different concentrations (100μg, 150μg, 200μg /disc) and the diameter of the zone of inhibition were recorded. The percentage increase in paw edema was reduced in both chloroform (18.20±1.32) and ethanol extract (24.22±1.12) treated animals when compared with standard Indomethacin (19.18±1.02) and control (40.10±1.22) animals. The chloroform extract possessed significant anti inflammatory activity that may be due to its ability to prevent the production of some pro-inflammatory mediators. The chloroform and ethanolic extract showed the inhibitory activity against all the four strains of organisms , but the ethanolic extract showed potent inhibition against Streptococcus pneumoniae and Staphylococcus aureus in higher concentration (200 μg/disc) when compared with Ciprofloxacin (10µg /disc).
M. Uvarani*, R. Arun Pandian, B. Jenila, R. Sutharsingh and A. Thangathirupathi
Department of Pharmacognosy, Sankaralingam Bhuvaneswari College of Pharmacy, Anaikuttam, Sivakasi-626130, Tamilnadu, India
20 April, 2012
02 July, 2012
27 July, 2012
01 August, 2012