ANTI-INFLAMMATORY, ANTIOXIDANT AND CYTOTOXICITY POTENTIAL OF METHANOLIC EXTRACT OF TWO BANGLADESHI BEAN LABLAB PURPUREUS (L.) SWEET WHITE AND PURPLEAbstract
This study was subjected to investigate in vitro anti-inflammatory, antioxidant and cytotoxic properties of methanol extracts of two Bangladeshi bean pods namely Lablab purpureus L. sweet ‘white’ and ‘purple’. Protease Inhibition method was used to determine in-vitro anti-inflammatory properties. DPPH free radical scavenging method, total flavonoids, Total antioxidants and reducing power of the two samples were assessed for antioxidant properties and cytotoxicity potentials were assessed by the brine shrimp lethality test.In in-vitro anti-inflammatory investigation there is a linear relation of %inhibition for the white bean pods which indicates having positive anti-inflammatory property. In DPPH test the lowest and highest IC50 values are 430.00µg/ml and 853.13μg/ml, with L. purpureus sweet ‘purple’ and L. purpureus sweet ‘white‘ respectively and compared with standard ascorbic acid. In Cytotoxicity test LC50 value was found 960.06 μg/ml for L. purpureus (L.) sweet ‘purple’ and 66.5 µg/ml for L. purpureus sweet ‘white’, So L. purpureus sweet ‘white’ is more potent. The total Flavonoid contents of the test samples are 42.55±5.77 and 32.09±0.36 mg/g quercetin equivalents for ‘white’ and ‘purple’ respectively. L. purpureus (L.) Sweet White and Purplemay posses’ potent anti-inflammatory, powerful antioxidant as well as good cytotoxic potential.
Mohammad Abdul Motalib Momin , Md. Razibul Habib*, Md. Rakibul Hasan , Jannatun Nayeem, Nizam Uddin , Md. Sohel Rana
Lecturer, Dept. of Pharmacy, East West University, Dhaka-1212, Bangladesh
12 September, 2011
12 February, 2012
20 February, 2012