ANTIBACTERIAL ACTIVITIES AND BRINE SHRIMP LETHALITY BIOASSAY OF THE CHLOROFORM EXTRACT OF STEM BARK OF CRATAEVA NURVALA BUCH HAMAbstract
Antibacterial and cytotoxic activities of the chloroform extract of the stem bark of Crataeva nurvala (Bengali name- Borun; Family- Capparidaceae) were studied. Antibacterial activity was determined against two Gram positive (Bacillus cereus, Bacillus megaterium,) and four Gram negative (Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shigella boydii) human pathogenic bacteria by disc diffusion method. The range of zone of inhibition of the extract was 8.3 to 22.1 mm. The extract showed no activity against Bacillus megaterium. Brine shrimp lethality bioassay method was used to determine the toxicity of the plant extract and ampicillin trihydrate was used as positive control. The LC50 values of standard ampicillin trihydrate and extract were 11.48 µg/ml and 14.12 μg/ml respectively. In addition serial dilution technique was used to determine the minimum inhibitory concentration (MIC) of the plant extract. The MIC values were recorded 62.5 µg/ml against both Escherichia coli and Bacillus cereus.
Shumaia Parvin, Md. Abdul Kader, Md. Ajijur Rahman*, Mir Imam Ibne Wahed and Md. Ekramul Haque
Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh
29 October, 2011
17 December, 2011
21 February, 2012