ANTIBACTERIAL ACTIVITY OF ANDROGRAPHIS PANICULATA OF METHANOLIC EXTRACT AGAINST SOME HUMAN PATHOGENIC BACTERIA AND EFFECT OF ANDROGRAPHOLIDE COMPOUND AGAINST BACTERIAL PATHOGENAbstract
Cold and hot methanolic extract of leaves and whole plant parts of Andrographis paniculata were tested against gram-negative Escherichia coli (MTCC 1679) Klebsiella pneumonia (MTCC 4032) Streptococcus pyogenes, Solmonella typhimurium (MTCC733) and gram-positive Staphylococcus aureus (MTCC737). Maximum activity among all the bacterial cultures tested was showed against E. coli MTCC1679 when compared with their standards. Next to E. coli, MTCC1679 best inhibition was seen against S. aureus (MTCC737). There was no inhibition reported against, K. pneumonia, S. pyogenes, Salmonella typhi. The studied result revealed the highest activity in 75% methanolic extract. The highest zone of inhibition (22 mm) was observed against E. coli at a dose of 3.0 mg. The antibacterial activities of 75% methanol extract from A. paniculata leaves were observed only against the E. coli. The extract was not found active against other tested bacterial pathogens. The Andrographolide compound was analyzed by HPLC chromatogram and the standard of the given andrographolide compound was also prepared by the same.
S. Ali * and S. A. Mir
Department of Post-Graduate Studies and Research in Biological Sciences, Rani Durgavati University, Jabalpur, Madhya Pradesh, India.
12 May 2019
25 November 2019
20 February 2020
01 March 2020