ANTIBACTERIAL ACTIVITY OF IN VITRO REGENERATED ROOTS OF BRYOPHYLLUM PINNATUM LAM. KURZAbstract
In vitro antibacterial activity of Bryophyllum pinnatum (in vivo generated and in vitro regenerated roots) were analysed in this study. Traditional uses for these plants treat specific conditions or diseases. The present study examined the antibacterial activity using the disk diffusion method using Ethanolic & Methanolic extract as part of the process of understanding the chemistry, toxicity and efficacy of these plant extracts. Methanolic and Ethanolic extracts of this plant was examined using a standard antimicrobial disk diffusion method. Extracts were tested against both Gram negative (Escherichia coli, Vibrio cholerae, Salmonella typhi and Pseudomonas aeruginosa) & Gram positive (Bacillus cereus & Staphylococcus aureus) bacteria. Methanolic in vitro regenerated roots showed higher inhibition zone against all bacterial species while natural growing plant root extract showed minimum inhibition. In vitro regenerated root extract showed moderate inhibition zone against all bacterial species. S. aureus showed maximum (25.2 mm) inhibition zone in in vitro root methanolic extract while V. cholerae showed minimum (14 mm) in natural growing root extract. In ethanolic extract maximum inhibition zone (22.1 mm) was observed against S. aureus and another maximum inhibition zone (21.3mm and 21mm) was reported against B. cereus and P. aeruginosa respectively. These results served to validate our procedures and indicate the need for the present study. Implications of these results for bioactivity and drug discovery potential of plant extracts can be further explored. This study serves as basis for further research on these weed plants.
Shinam Mukhija *, Chandra Gurnani and Vikram Kumar
Department of Biotechnology, IASE (D) University, Sardarshahar, Churu, Rajasthan, India
22 May, 2016
19 July, 2016
27 July, 2016
01 October 2016