ANTIBACTERIAL ACTIVITY OF SIMAROUBA GLAUCA LEAF EXTRACTS AGAINST FOOD BORNE SPOILAGE AND PATHOGENIC MICROORGANISMSAbstract
Crude ethanol and methanol extracts from dried and fresh leaves of Simarouba glauca were tested for their inhibitory activity against two food borne pathogenic microorganisms (Staphylococcus aeureus and Escherichia coli) and two food spoilage microorganism (Bacillus subtilis and Pseudomonas aeurogenosa). Screening for antimicrobial activity using well diffusion assay showed the inhibition against entire tested microorganisms. On the other hand the maximum zone of inhibition was recorded of fresh leaves methanol extract (FLM) about 11 mm against Escherichia coli and the lowest zone of inhibition was recorded of fresh leaves methanol extract (FLM) about 2 mm against Bacillus subtilis. Minimum inhibitory concentrations (MIC’s) of extracts were determined using agar dilution method on the same test microorganisms. Fresh leaves methanol (FLM) extract gave MIC value ranging from 160 to 10,240 parts per million (ppm). Result showed that the Bacillus subtilis was the most sensitive microorganism.
B. L. Jangale*, T. B. Ugale , P. S. Aher , N. R. Toke , A. N. Shivangikar and N. T. Sanap
Assistant Professor, Department of Plant Biotechnology, K. K. Wagh College of Agricultural Biotechnology, Saraswati Nagar, Panchavati, Nashik- 422 003, Maharashtra, India
30 September, 2011
09 November, 2011
29 January, 2012