ANTIBACTERIAL ACTIVITY OF THREE URTICA SPECIESAbstract
The antimicrobial activity of Urtica dioica L. and Urtica urens L. has been reported; however, there are no reports regarding Urtica mexicana Liebm., which is widely distributed in central Mexico. U. urens and U. mexicana were collected in Amecameca and U. dioica was collected in Toluca Valley, both located in the state of Mexico. Crushed leaves of the plants were soaked consecutively in hexane, dichloromethane, methanol and water. The minimum inhibitory concentration (MIC) was determined with 0.039 mg/mL to 5 mg/mL solutions of the extracts, which were added to cultures of Salmonella typhimurium, Shigella flexneri, Salmonella typhi, Escherichia coli, Proteus mirabilis, Bacillus subtilis, and Staphylococcus aureus (5 × 106 CFU/mL) with Resazurin sodium salt and Mueller‒Hinton agar. The lowest MIC (0.039 mg/mL) was observed for the hexane, dichloro-methane, and the methanol extracts of U. urens against Bacillus subtilis and the hexane and dichloromethane extracts of U. urens against S. aureus (0.156 mg/mL). U. mexicana showed a greater spectrum of activity at higher concentrations; U. dioica showed exhibited the least activity. U. mexicana, U. urens and U. dioica produced 14, 15 and 7 inhibitory extracts, respectively. The plants collected in Amecameca demonstrated higher antibacterial activity.
R. V. Lezama *, R. T. Aguilar, J. R. Calvo, T. E. S. Siles, S. B. Herrerasolis, R. Cerón and J. S. Carrillo
Department of Health Sciences, Universidad Autónoma Metropolitana (UAM, Metropolitan Autonomous University Mexico City, Mexico.
02 January 2020
03 June 2020
17 June 2020
01 February 2021