ANTIBACTERIAL AND CYTOTOXIC ACTIVITIES OF THYMUS VULGARIS LEAVES GROWN IN OMANAbstract
Thyme (Thymus vulgaris L.) is a pleasant smelling perennial shrub, which grows in several regions in the world. The plant is reported to have high economic and medicinal values. The objectives of this research was to determine antimicrobial activity of petroleum ether, chloroform and hydroalcoholic extracts obtained from leaves of Thymus vulgaris collected from Oman. The other objective was to determine cytotoxic activity of the three extracts. Antimicrobial activity was measured using disc diffusion method against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia. Brine shrimp test was used to estimate cytotoxic activity. In antibacterial assay, Thymus vulgaris leaves extracts showed very strong results, inhibition zones ranged from 7 – 20 mm. So this plant can be used as a good source of potential antimicrobial agent.Petroleum ether showed the highest antibacterial activity. Furthermore, Petroleum ether and Chloroform extracts have almost killed all the shrimp larvae at higher concentration of 1000 mcg/ml. LC50 values for the two extracts were 85.2 and 95.8 mcg/ml, respectively. Polar fractions like hydro alcoholic extract have displayed very low cytotoxic activities.
Aisha Haroon Al-Balushi, Maryam Aljabri, Md. Sohail Akhtar*, Sadri Said, Afaf Weli, Qasim Al-Riyami and Ahlam A. AlAbri
School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, Nizwa, Sultanate of Oman
20 June, 2013
10 September, 2013
10 October, 2013
01 November, 2013