ANTIBACTERIAL AND CYTOTOXIC ACTIVITY OF JUNIPERUS INDICA BERTOL FROM NEPALESE HIMALAYAAbstract
Antimicrobial and cytotoxic activity of petroleum ether, ethyl acetate and methanol extracts from Juniperus indica Bertolwere evaluated. The extracts screened for their antibacterial activity against human pathogenic bacterial strains, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. All the fractions presented antimicrobial activity with variable strengths to all of the tested microorganisms. Phytochemical screening revealed the presence of terpenoids, flavanoids and tannins as main phytochemical groups. Cytotoxic activity was evaluated by using brime shrimp lethality test. LC50 value for the methanolic, ethyl acetate, and petroleum ether extracts were calculated at 550 µg/ml, 380 µg/ml and 140 µg/ml respectively.
Binita Mahajan, Tirtha Maiya Shrestha and Rajendra Gyawali*
Department of Pharmacy, Kathmandu University, Dhulikhel, Nepal
17 December, 2011
10 January, 2012
25 March, 2012
01 April, 2012