ANTIBACTERIAL, ANTIFUNGAL AND PHYTOTOXIC SCREENING OF SOME PREPARED PYRAZINE DERIVATIVES IN COMPARISON TO THEIR RESPECTIVE α- DIKETO PRECURSORSAbstract
Antibacterial, antifungal and phytotoxic activities of the prepared pyrazine derivatives of benzil (A), 4, 4’-dibromobenzil (B) and α-diketones of triterpenoids skeleton e.g. 2, 3-diketolupene (C) and 2, 3-diketomethyl- dihydrobetulonate (D) were studied. The prepared 1, 4-pyrazine derivatives (E, F, G & H respectively) have been characterized by UV, IR, NMR (1H and 13C), optical rotation, mass spectra and by comparison with authentic samples. Disc diffusion technique was used to determine in vitro antibacterial and antifungal activities. Phytotoxicity was determined against seed growth technique. In addition, minimum inhibitory concentration (MIC) was determined using serial dilution technique to determine antibacterial potency. The derivatives showed significant antimicrobial activities against Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa (bacterial specimen), Colletotrichum camelliae, Fusarium equisiti, Alternaria alternata, Curvularia eragrostidis and Colletotrichum gloeosporioides (fungal specimen).
Pranab Ghosh*, Prasanta Chakraborty and Goutam Basak
Department of Chemistry, University of North Bengal, Dist. Darjeeling-734013, West Bengal, India
26 March, 2011
21 May, 2011
28 June, 2011
01 July, 2011