ANTIBACTERIAL, RADICAL- SCAVENGING ACTIVITIES AND CYTOTOXICITY PROPERTIES OF PHALERIA MACROCARPA (SCHEFF.) BOERL. LEAVES IN HEPG2 CELL LINESAbstract
Antibacterial, and radical scavenging activities of four crude extracts of Phaleria macrocarpa (Scheff) Boerl.) leaves extracts and its active crude cytotoxic properties in HepG2 cells were investigated. The susceptibility bacteria tests were performed by the Mueller Hinton agar well diffusion method. The Radical scavenging activity was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method, while antioxidant potency was tested by DPPH TLC bioautographic assay. The cytotoxic was measured by the MTT assay against human hepatoma cell lines (HepG2). Crude extract MMD showed an equally good activity against Pseudomonas aeroginosa, and strong activity against Bacillus cereus and Streptococcus aureus, with the inhibition zone having diameter between 15-18mm. Crude extract EMD showed has activity against all bacteria compare others, an equally good activity against Escherichia coli, Klebsiella pneumonia and Streptecoccus ubellis, and has strong activity against Pseudomonas aeroginosa, Bacillus cereus and Streptococcus aureus, with the inhibition zone having diameter between 15mm – 27mm. The lowest activity showed for HMD and CMD (diameter<10mm). Crude extract of MMD and EMD exhibited a strong and moderate radical scavenging with the inhibition percentages are 79% and 76% respectively. Crude extract of HMD and CMD exhibited a low free radical scavenging with the inhibition percentages in the range between 59-69%. EMD and MMD extracts exhibited low mildly cytotoxic effect against HepG2 cell line giving IC50 values between 30-60 µg/mL.
Andriani Yosie M. A. W. Effendy*T. M. T. Sifzizul and Mohamad Habsah
Director, Institute of Marine, Biotechnology, Universiti Malaysia Terengganu, Terengganu, Malaysia
28 March, 2011
26 May, 2011
28 June, 2011
01 July, 2011