ANTIDIABETIC AND ANTI-INFLAMMATORY EFFECT OF PROBIOTIC LACTOBACILLUS PARACASEI MCC3195 IN HIGH FRUCTOSE FED TYPE 2 DIABETIC RATS

The aim of this study was to investigate the antidiabetic and anti-inflammatory effects of probiotic Lactobacillus paracasei MCC3195 in high fructose fed type 2 diabetic rats. High fructose diet was used to induce type 2 diabetes in male albino wistar rats. L. paracasei MCC3195 was administered to rats at a single dose of 2×10⁸ cfu/ml per rat for 8 week. The body weight, fasting blood glucose, oral glucose tolerance test, glycosylated hemoglobin, insulin, Liver glycogen level, lipid profile and oxidative stress parameters were analyzed. Interlukin-6 and Interlukin-10 were also estimated in serum. Histopathology analysis of pancreas and liver injury were done. At 8 week body weight, FBG, glucose tolerance, HbA1c, insulin, liver glycogen level, total cholesterol, triacylglycerol, LDL-C, VLDL-C, lipid peroxidation and IL-6 were significantly increased in diabetic rats, while administration of L.  paracasei MCC3195 significantly decreased these factors. The level of HDL-C, reduced glutathione, superoxide dismutase, catalase and IL-10 were significantly decreased in diabetic rats, whereas administration of L. paracasei MCC3195 significantly increased these factors. Administration of L. paracasei MCC3195 protects the Islets of langerhans and hepatic cells from destruction as compared to the diabetic rats. Probiotic L.  paracasei MCC3195 exerts the antidiabetic and anti-inflamatory effect in high fructose-fed type 2 diabetic rats, by significantly improving glucose intolerance, hyperglycemia, hyperinsulinemia, dyslipidemia, oxidative stress and immunoregulatory properties as well as protects the type 2 diabetes induced complications. Further research is needed in other diabetic models for clinical application of L.  paracasei MCC3195 in delay or prevention and progression of T2DM.