ANTIGENOTOXIC EFFECTS OF SILYMARIN AGAINST NICKEL CHLORIDE MEDIATED DNA DAMAGE IN CULTURED HUMAN PERIPHERAL BLOOD LYMPHOCYTESAbstract
DNA damage induced by Nickel chloride in lymphocytes has been studied in-vitro. The sister chromatids exchange and single-cell gel electrophoresis (SCGE), or comet assay were used to measure the level of DNA damage in terms of SCE frequency and comet parameters. Silymarin is a natural protective compound, isolated from Silybum marianum seeds and reported for its anti-genotoxic properties. In this study, the peripheral blood lymphocytes were administrated along with silymarin and Nickel chloride (NiCl2) to evaluate the antigenotoxic effect of silymarin against NiCl2 mediated genotoxicity. A significant (P<0.05) increase in the tail moment and SCE frequency indicating DNA damage was observed in NiCl2 (129.59 µg/mL) treated samples. Simultaneously, a gradual decrease was observed in the tail moment, and SCE frequency in silymarin-treated samples against NiCl2 induced DNA damage. Results showed that silymarin at a concentration of 40 µg/mL against NiCl2 induced DNA damage showed a significant (P< 0.05) decrease in genotoxicity.
Veena Vishwakarma, Sunil Kumar, Bharti Yadav, Ranjan Gupta, Neeraj K. Aggarwal and Anita Yadav *
Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India.
23 October 2020
15 February 2021
24 May 2021
01 October 2021