ANTIHYPERGLYCEMIC AND ANTIOXIDATIVE EFFECT OF HYDRO-METHANOLIC EXTRACT OF LEAF OF ABROMA AUGUSTA IN STREPTOZOTOCIN INDUCED DIABETIC MALE ALBINO RAT

The present study was conducted to investigate protective effect of hydro-methanolic (2:3) extract of leaf of Abroma augusta in streptozotocin induced diabetic male albino rat. For this purpose animals were divided into three treatment groups (n = 6): control group, STZ induced diabetic group (STZ 4 mg / 0.1 ml citrate buffer/ 100 g body weight), hydro-methanolic extract of leaf of Abroma augusta treated group (20 mg / 0.5 ml distilled water/100 g body weight). Treatment was conducted for 28 days. Blood glucose, serum insulin, glycated haemoglobin levels along with activities of hepatic carbohydrate metabolic enzymes, hexokinase and glucose-6-phosphate dehydrogenase were assed in all experimental groups. Oxidative stress markers like catalase, peroxidase enzyme activities, conjugated diene and thiobarbituric acid reactive substance levels in liver were measured. All these parameters were significantly recovered towards the control level after the treatment of hydro-methanolic extract. Pancreatic islet diameter, count of islet and number of cells in islets also decreased in diabetic animals. Treatment of these animals with extract resulted significant recovery towards the control level. Serum glutamate oxaloacetate transaminase and glutamate pyruvate transaminase were assed in all the groups and it has been revealed that the said extract has no metabolic toxicity in general. From this study it may be concluded that hydro-methanolic extract of leaf of A. augusta has protective effects on diabetes induced complications.