ANTIHYPERTENSIVE ACTIVITY GUIDED-FRACTIONATION OF NARDOSTACHYS JATAMANSIAbstract
The present study aimed to examine the anti-hypertensive constituents present in Nardostachys jatamansi. Crude solvent extracts (dichloromethane; ethyl acetate; methanol, and water) of this plant rhizomes based on the difference in polarity were prepared and tested for inhibition of angiotensin-converting enzyme (ACE). Preliminary phytochemical analysis showed bioactive compounds such as alkaloids, flavonoids, carbohydrates, triterpenoid, glycosides, saponin, and tannins in different extracts. Ethyl Acetate extract has shown better results with 93% inhibition at 100 µg concentration as compared to other solvent extracts. Fractionation of ethyl acetate extract over silica gel column chromatography with hexane and ethyl acetate under gradient elution resulted in six fractions. However, on purification of the ethyl acetate extract, ACE inhibition activity diminishes with only 19.03 to 47.35% inhibition for the purified fractions. Nardostachys jatamansi can be used to treat hypertension-related disorders, and ethyl acetate extract can be further investigated to identify potent molecules that work in a synergistic combination.
B. B. Rachotimath, M. S. Gavimath and B. Padmashali *
Department of Chemistry, School of Basic Sciences, Rani Channamma University, Belagavi, Karnataka, India.
28 April 2020
04 September 2020
11 September 2020
01 May 2021