ANTIMICROBIAL ACTIVITIES AND PRELIMINARY PHYTOCHEMICAL SCREENING ON THE CRUDE EXTRACTS OF THE LEAVES OF CINERARIA ABYSSINICA SCH. BIP. EXA. RICHAbstract
In Ethiopian traditional medicine, the aqueous decoction of the leaves of Cineraria abyssinica Sch. Bip. exA. Rich (Asteraceae) is used for treatments of various ailments including diarrhea, however, to date, there appear to have been no reports on the phytochemistry and the antimicrobial activity of the plant. The main aim of this study was, therefore, to carry out preliminary phytochemical screening and antimicrobial activities on leaf extracts of C. abyssinica. The in vitro antimicrobial activities of the aqueous and 80% methanolic crude extracts of the leaves of C. abyssinica were investigated against Gram positive and Gram negative bacteria by agar disc diffusion method. Both the aqueous and 80% methanolic extracts showed various degrees of potent antibacterial activities comparable to the standard drug ciprofloxacin against all of the bacteria tested except Bacillus species. Preliminary phytochemical screening of the plant revealed the presence of polyphenols, flavonoids, coumarins, saponins, terpenoids and phytosterols. The acute toxicity studies showed the nontoxic nature of the plant up to 3 g/kg. Therefore, the present study revealed for the first time the presence of antimicrobial phytochemicals in the leaves of C. abyssinica that scientifically validated the traditional use and its great potential to be used for treatment of infectious diarrhea.
Biruk Sintayehu*, Kaleab Asres and Avijit Mazumder
Lecturer, B. Pharm, M. Sc. (Pharmacognosist), Pharmacognosy Course and Research Unit, Department of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, Ethiopia
31 January, 2012
14 March, 2012
28 April, 2012
01 May, 2012