ANTIMICROBIAL ACTIVITY OF AMYCOLATOPSIS ORIENTALIS JAR10 FROM AGRICULTURAL FIELDAbstract
Actinomycetes are versatile and prolific source of microbial natural products with great advantage in medicine and agriculture. Recently there is growing interest in exploring biological active compounds from rare actinomycetes. Usually rare actinomycetes are regarded as strains of actinomycetes whose isolation frequency by conventional methods is much lower than that of Streptomycetes strains. Amycolatopsis orientalis JAR10 rare genera of actinomycetes which have been isolated, characterized and antimicrobial activity against various pathogens have been determined in the present work. The morphological, cultural, genotypical and physiological characteristics of strain JAR10 has been examined by International Streptomyces Project. The bioactive metabolites produced by strain JAR10 were extracted by solvent extraction method and the crude extract obtained was further analyzed against various pathogens. The minimum inhibitory concentration of crude extract obtained from Amycolatopsis orientalis JAR10 was analyzed by broth dilution method and was found to inhibit Staphylococcus aureus at 60 µg/ml indicating the presence of biological active compounds.
Ritika Chauhan and Jayanthi Abraham*
Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India.
18 June, 2014
28 August, 2014
13 November, 2014
01 February, 2015