ANTIMICROBIAL ACTIVITY OF MENTHA ARVENSIS AGAINST CLINICAL ISOLATES OF HUMAN CARIOGENIC PATHOGENS- AN IN-VITRO STUDYAbstract
Patients with chronic dental infection are usually treated with antibiotics. However, the value of antibiotics was decreasing because increased resistance in bacteria. The objective of this study is to evaluate the efficacy of herbal crude extract of Mentha arvensis in human Cariogenic pathogens. In this study we obtained crud extract of Mentha arvensis in different solvent 50% and 10% methanol, ethyl acetate, chloroform and was tested against human Cariogenic pathogens Streptococcus mutans, Streptococcus sangunis, Staphylococcus aurues, Lactobacillus casei were isolated from patients having dental disease. The crude extracts activity were studied by disc diffusion and both dilution methods in different concentration. Studies were also undertaken to assess the phytochemical composition of the Mentha arvensis extract. 50% methanolic extract at 2.5mg/ml and 5mg/ml concentration shows slightly bigher zone of inhibition (ranging from 26 to 30 mm and 28 to 32 mm), and 10% methanolic 2.5mg/ml and 5mg/ml extract shows slightly small zone (ranging from 20 to 24 mm and 22 to 27 mm) and comparison with ethyl acetate and chloroform shows small zone at 5mg/ml ranging from 15 to 18 mm and 13 to 17 mm and in 2.5gm/ml ranging from 14 to 15mm and 09 to 16 mm or to be moderately sensitive. MIC results exhibit the profound and promising activity of Mentha arvensis on BHI 0.090 mg/ml. The secondary metabolites commonly present in the test leaves are Alkaloids, Tannins, Flavonols, Steroids, Xantones and glycosides, The GCMS analysis of revealed, the presence of Eucalyptol, Isomethone, Linalool, methnol, 4-Terpineol, OleicAcid, Tetradecanoic acid, 12-methyl-, methyl ester, Hexadecanoic acid, (Palmitic acid) methyl ester. These data suggest that extracts of Mentha arvensis contain significant amounts of phytochemicals with antioxidative properties which could serve antimicrobial property of the Mentha arvensis and it is exploited as a potential source for plant-based pharmaceutical products. These results could form a sound basis for further investigation in the potential discovery of new natural bioactive compound.
Deepak Dwivedi, Gaurav Khandelwal , Rakesh Kumar Patidar and Vinod Singh*
Associate Professor & Head, Department of Microbiology, Barkatullah University, Hoshangabad Road, Bhopal, Madhya Pradesh, India
06 January, 2012
16 February, 2012
19 April, 2012
01 March, 2012