ANTIMICROBIAL ACTIVITY OF PRICKLY CHAFF (ACHYRANTHES ASPERA) SEED TRYPSIN INHIBITORAbstract
Trypsin inhibitor (AATI) was purified from the seeds of Achyranthes aspera to homogeneity by conventional methods and its antimicrobial activity was tested on selected pathogenic microbes. The inhibitor significantly affected the growth of Proteus vulgaris followed by Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumonia with zones of inhibition recorded as 28mm, 26mm, 25mm, 20mm and 14mm respectively. No inhibition, however, was observed with Staphylococcus pneumonia strain. Minimum inhibitory concentration (MIC) values of AATI supported that Proteus vulgaris and Bacillus subtilis were the most sensitive strains against the inhibitor. AATI did not differentiate gram positive and gram negative bacteria in its antibacterial activity. The trypsin inhibitor was ineffective against fungal strains Asperigillus niger, Fusarium oxysporum, Alternaria alternate and Candida albicans tested.
Geetha Konala, Lavanya Seva and Siva Prasad Davuluri*
Department of Biochemistry, Andhra University, Visakhapatnam-530 003, Andhra Pradesh, India
26 February, 2012
16 August, 2012
28 August, 2012
01 September, 2012