ANTIMICROBIAL ACTIVITY OF SUPERCRITICAL CARBON DIOXIDE EXTRACTS OF TUBEROSE (POLIANTHES TUBEROSA LINN.) FLOWERS AGAINST COMMON PATHOGENSAbstract
Tuberose flower extracts were obtained by green technology of Supercritical carbon dioxide (SC-CO2) extraction and solvent extractions (using n-hexane and petroleum ether of b.p. 60-80 °C). Antimicrobial activities of these extracts were tested in vitro against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Helicobacter pylori, Vibrio cholerae and Candida albicans. No activity was found against H. pylori and V. cholerae. Antimicrobial activities of the extracts were established by minimum inhibitory concentration (MIC) assays against the remaining test microorganisms. The SC-CO2 and solvent extracts showed potency against all test microorganisms. SC-CO2 extract obtained at 40 °C, 100 bar pressure, 135 min extraction time, and n-hexane extract showed most promising results, among all the extracts. However, considering the global need for green extracts for therapeutic applications, SC-CO2 extraction is the recommended technique for extracting antimicrobials from tuberose flowers. The chemical constituents conferring antimicrobial potencies to the extracts were also identified by GC/MS analyses.
Probir Kumar Ghosh , Paramita Bhattacharjee* and Satadal Das
Assistant Professor, Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata 700032, India
28 October, 2013
04 January, 2014
09 March, 2014