ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF CHLAMYDOMONAS REINHARDTII SP.

The present investigation was undertaken to study the antimicrobial and antioxidant activity in algae (Chlamydomonas reinhardtii CC 124).  The extractions of metabolites were done by two methods (glass bead and steel ball) using methanol: chloroform: water (5:2:2) as solvents.  The presences of metabolites were identified by thin layer chromatography and structural analysis was done by Gas Chromatography and Mass Spectrometry. Antimicrobial activities of the extracts were assessed against bacterial and fungal strains (Bacillus subtilis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Candida albicans, Aspergillus niger, Aspergillus flavus) by disc diffusion method and compared with standard antibiotics.  The results, of both extracts showed more potent antimicrobial activity in (B. subtilis,P. aeruginosa, K. pneumoniae) bacterial species and (C. albicans) fungal strain. The antioxidant activity was tested by DPPH (2, 2-Diphenyl-1-picrylhydrasyl) free radical method and determination of H₂O₂ radical scavenging activities were also performed. The presence of antioxidants was compared with commercial antioxidants, such as BHT (Butylated Hydroxy Toluene), BHA (Butylated Hydroxyl Anisole).The phenolic content was compared with gallic acid. From the present study, it can be concluded that the two different extracts of the algae has the antimicrobial and antioxidant activity.