ANTIMICROBIAL AND CYTOTOXIC ACTIVITY ASSESSMENT OF THE AQUEOUS METHANOLIC AND PET-ETHER EXTRACT OF THE LEAVES OF MESUA FERREAAbstract
The purpose of the study is to evaluate the antimicrobial and cytotoxic activity of methanolic and pet-ether extract of leaves of Mesua ferrea. The antimicrobial activity was investigated against eight pathogenic Gram-positive and Gram-negative bacterial species at three concentrations (100, 300, 500 µg/disc) by using disc diffusion method. Both extracts showed moderate antibacterial activity against six bacterial species among eight and the activity was increased with increasing concentration of the sample. The maximum antimicrobial potential was obtained against Bacillus subtilis, Bacillus megaterium and Staphylococcus aureus in case of both extracts. The cytotoxic effect of the extracts was assayed on Artemica salina leaches. The LC50 values were found to be 7.94 μg/mL for methanolic extract and 3.98 μg/mL for pet-ether extract compared to 0.451 μg/mL for standard drug vincristine sulphate. The study confirms that the plant extracts have moderate antimicrobial activity and considerable amount of cytotoxic activity.
H.M. Arif Ullah*, Syed Mohammed Tareq , Irfanul Huq , Muhammad Borhan Uddin and Md. Salauddin
Lecturer, Department of Pharmacy, North South University, Dhaka, Bangladesh
14 April, 2013
21 July, 2013
28 August, 2013
01 September, 2013