ANTIMICROBIAL AND CYTOTOXIC ACTIVITY OF ASTERACANTHA LONGIFOLIA NEES.Abstract
The antibacterial and antifungal activity of methanolic extract of Asteracantha longifolia Nees has been evaluated against 4 Gram positive bacteria, 7 Gram negative bacteria and 7 fungi. Ciprofloxacin and fluconazole used as standards for bacteria and fungi, respectively. The extract showed varying degrees of antimicrobial activity with zone of inhibition ranging from 15.0 to 26.0 mm. A. longifolia demonstrated significant zone of inhibition against all experimental bacteria, namely, B. cereus, B. megaterium, B. subtilis, S. aureus, E. coli, P. aeruginosa, S. paratyphi, S. typhi, Sh. dysenteriae, Sh. Sonnei and V. cholerae and four fungi, namely, A. niger, B. dermatitidis, Microsporum spp. and Trichophyton spp. The MICs of the plant extract was found to be 31.25 μg/ml against B. cereus, S. typhi, Sh. Sonnei, V. cholerae, A. niger and B. dermatitidis. In the brine shrimp lethality bioassay, the LC50 and LC90 of A. longifolia were found to be 6.1 & 12.2 μg/ml, respectively.
Md. Hassan Kawsar*, Md. Akbar Hossain , Aninda Kumar Nath , Md. Zia Uddin , Mycal Dutta and Md. Masud Morshed
Associate Professor, Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh
07 July, 2012
03 November, 2012
24 November, 2012
01 December, 2012