ANTIMICROBIAL PROFILE OF RHODOCOCCUS ERYTHROPOLIS VL-RK_05 ISOLATED FROM MANGO ORCHARDSAbstract
The aim of the present study was to isolate, identify and to analyze the phylogenetic characteristics of the potent actinobacterial strain VL-RK_05 isolated from Mango orchards of Vissannapet, Krishna Dist, Andhra Pradesh. Soil samples collected were pre treated with calcium carbonate and used for isolation of potent Actinobacterial strain designated as VL-RK_05. Identification of the strain was carried out by studying the micro morphological, cultural, biochemical and physiological methods. The Phylogenetic study of the strain was carried out by employing 16S r RNA sequence based analysis. Phylogenetic tree was constructed using the Molecular Evolutionary Genetic analysis version 5. Phylogenetic analysis of 16S r RNA sequencing revealed that the strain is closely related to Rhodococcus erythropolis and the bioactive metabolites produced by the strain inhibited Gram positive bacteria (Staphylococcus aureus, Bacillus megaterium), Gram negative bacteria (Shigella flexneri, Xanthomonas campestris, Proteus vulgaris, Pseudomonas aeruginosa and Escherichia coli) and fungi Aspergillus niger, Botrytis cinerea, Fusarium solani F. oxysporum and Candida albicans.
Rajesh Kumar Munaganti, Krishna Naragani and Vijayalakshmi Muvva*
Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India.
01 August, 2014
15 October, 2014
16 December, 2014
01 April, 2015