ANTIMICROBIAL PROPERTIES OF DIFFERENT SOLVENTS EXTRACT OF RUMEX VESICARIUS LINN. ON SOME SELECTED BACTERIAL AND FUNGAL ISOLATESAbstract
The Antimicrobial (antifungal and antibacterial) properties of the different solvents extracts (benzene, pet ether, acetone, chloroform, methanol and aqueous) of Rumex vesicarius Linn plant plants, on four bacterial (Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) and four fungal isolates (Aspergillus niger, Tricoderma reesei, Penicillium funiculosum and Fusarium oxysporum) were investigated by well diffusion assay. The minimum inhibitory concentration (MIC) of these solvents extracts evaluated at different concentration (3.25 mg/ml, 6.25 mg/ml, 12.5 mg/ml, 25 mg/ml and 50 mg/ml) for the tested organisms. The widest spectrum of antimicrobial activity was recorded for pet ether extract of leaf against P. funiculosum (30±1.2mm), F. oxysporum (22±0.8mm), E. coli(20±0.9mm) and P. Aeruginosa (10±0.7mm). This was followed by the benzene extract of leaf, which inhibits P. funiculosume t4l(20±0.8mm), F. oxysporum (18±0.4mm), E.coli(10±0.7mm). Acetone extract of flower work against P. funiculosum (30±1.4mm), A. niger, (12±0.7mm), E. coli(20±0.6mm) and S.aureus (18±0.8mm). The inhibitory effect of chloroform extract of flower against A.niger, (18±0.3mm) and F. oxysporum (16±0.3mm). The antimicrobial activity of methanolic and aqueous extract of plant against the organisum was minimal as compared to others.
Ankita Shah*, Parul Sharma and Rekha Vijayvergia
Plant Pathology and Plant Biochemistry Laboratory, Department of Botany, University of Rajasthan, Jaipur-302004, India.
14 July, 2014
06 September, 2014
19 January, 2015
01 March, 2015