ANTIOXIDANT ACTIVITY OF LEAVES SOLVENT EXTRACT OF MIMUSOPS ELENGI LINN.Abstract
The present study was to estimate the total phenolic content, flavonoids content and evaluate the in vitro antioxidant activity of alcoholic and aqueous extract of the leaves of plant Mimusops elengi Linn. Total phenolic content was determined calorimetrically using Folin ciocalteu reagent, and Total flavonoid content was determined by aluminum chloride method. The total phenolic content of methanol, ethyl acetate soluble fraction, and aqueous extract was found 23.22, 22.44, and 15.88%w/w respectively. In the case of total flavonoid content was found 33, 31, and 24%w/w respectively. Antioxidant activity was measured based on the DPPH radical scavenging assay, Nitric oxide scavenging assay, and reducing power assay. A methanol extract of the leaves of the plant showed potent free radical scavenging activity with an IC50 value of 65.00 μg/ml. However, standard ascorbic acid activity was significantly higher than that of all extracts. The IC50 value of the standard (Ascorbic acid) was 7.779μg/ml. In the case of nitric oxide scavenging assay, the methanolic & aqueous extract of the leaves of the plant Mimusops elengi showed a potential antioxidant effect. For reducing power assay, all extracts showed an increase in absorbance with an increase in concentration. Because the highest reducing power was observed with the methanol extract of the leaves. However, it may be due to the presence of the highest total antioxidant content of this extract that is mg of ascorbic acid equivalent per gram of plant extract, which is a potent reducing agent.
A. Asif *, S. Ahmad, M. R. M. Usman, T. Shaikh, M. Husain and Z. Shaikh
Department of Pharmacognosy, Gangamai College of Pharmacy, Nagaon, Dhule, Maharashtra, India.
03 April 2020
07 July 2020
16 August 2020
01 April 2021