ANTIOXIDANT AND ANTIBACTERIAL POTENTIAL OF MURRAYA KONIGII AGAINST HUMAN CARIOGENIC PATHOGENSAbstract
This study was to evaluate the antioxidant and antibacterial efficacy of herbal crude extract of Murraya koenigii againsthuman Cariogenic pathogens. In this study Methanolic extract of Murraya koenigii were studied for phytochemical composition and GC-MS for active compound. Antioxidant potential assay by 1,1-Diphenyl- 2-picrylhydrazyl (DPPH), 2, 2’-azinobis-3 ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assays. Further extracts were studied for antibacterial activity. Methanolic extract of Murraya koenigii revealed the presence of terpenoids, alkaloids, tannins, steroids, and saponins. Reducing ability of extracts was in the range (in μm Fe(II)/g) of 3176.98±63.87 were closed to quercetin 3208.27 ± 31.29. A significant inhibitory effect of extract (IC50 = 0.019 ± 0.69 mg/ml) on ABTS free radicals was detected. The antioxidant activity of the extract (96%) DPPH radicals were comparable with that of ascorbic acid (98.8%) and BHT (97.5%). Antibacterial effect of 50% and 10% methanolic extracts was detected on Cariogenic pathogens as compared with standard drug Amoxicillin. GC-MS results revealed the presence of hexadecanoic acid, linoleic acid, oleic acid, propanoic acid, acetophenone. Extracts of Murraya koenigii contain significant amounts of phytochemicals, antioxidant and antibacterial property and it is exploited as a potential source for natural medicine.
Deepak Dwivedi*, Rakesh Kumar Patidar and Vinod Singh
Scientist Microbiology, Minor Forest Produce Processing & Research Center, Barkheda Pathani, Bhopal, M.P. Pincode-462021, India
24 May, 2012
20 July, 2012
29 August, 2012
01 September, 2012