ANTIOXIDANT AND ANTILEUKEMIC ACTIVITY OF VITEX NEGUNDO LEAF EXTRACTSAbstract
Vitex negundo is an Indian medicinal plant used to cure several diseases such as asthama, fever, headache, cancer and many other diseases. This study aims to determine the antioxidant potential and anti-proliferative activity of Vitex negundo methanolic leaf extract. Isolation of bioactive compounds involved liquid-liquid extraction of methanolic leaf extract by petroleum ether and chloroform. The total antioxidant potential of the extracts was determined using phosphomolybdenum, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Ferric oxide reducing power (FRAP) and Nitric Oxide methods. It was observed that the crude and chloroform extracts had good antioxidant activity. The antileukemic activity of the chloroform extract was tested on HEL92.1.7 and Jurkat cell lines (Acute lymphoblastic leukemia derived) by cytotoxicity assay (MTT). The plant extracts exhibited poor cytotoxicity against the erythroid cell lines but showed good cytoxicity on Acute lymphoblastic leukemia cells. This study shows that the chloroform fraction of V negundo leaf has both antioxidant and antileukemic activity.
G. N. Shubha *, K. N. Shanti, Rukmini Jonnalagadda, Richa Kamboj and Sucheta Sridhar
Department of Biotechnology, PES Institute of Technology, Bangalore, Karnataka, India
22 June, 2016
22 July, 2016
02 August, 2016
01 December, 2016