ANTIOXIDANT AND BRINE SHRIMP LETHALITY BIOASSAY OF PECTIN FROM GRAPE FRUIT (CITRUS PARADISI) AND LEMON (CITRUS LIMON)Abstract
Pectin is a complex polysaccharide and has been used in food and pharmaceutical industries. The current study aimed to determine antioxidant and cytotoxic activities of pectin from peels of grape fruit (GP) and lemon (LP) by ferric reducing power (FRP) assay and brine shrimp lethality bioassay respectively. In addition, antioxidant and cytotoxic activities of GP and LP were compared with food-grade pectin (FGP). GP and LP exhibited positive results of antioxidant and cytotoxic activities. The percentage FRP of GP at 10 to 100 µg/ml was found to be higher as compared to FGP while LP showed closed values to that of FGP. The observed percentage mortality of shrimps at 10 to 1000 µg/ml by LP was found to be higher as compared to percentage mortality caused by FGP. Percentage mortality of shrimps by GP and FGP showed closed values. The LC50 of LP was found to be lower than that of FGP but close to the LC50 of standard drug etoposide. The LC50 of GP was closed to that of FGP and higher than that of etoposide. The obtained results suggested cost-effective use of GP and LP for antioxidant and cytotoxic actions.
S. Qadir, N. H. Siddiqui, A. Ahmed, I. Azher and Z. A. Mahmood *
Department of Pharmacognosy and Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan.
23 April 2019
26 September 2019
06 November 2019
01 February 2020