ANTIOXIDANT MEDIATED ULCER HEALING POTENTIAL OF HYPERIN ISOLATED FROM FLOWERS OF RHODODENDRON ARBOREUM IN EXPERIMENTAL RATAbstract
Hyperin, a flavonoid is an active phytochemical constituent gift in numerous plants as well as, Hypericum perforatum, Drosera rotundifolia, Stachys byzantina, Prunella vulgaris, Rumex acetosella, Abelmoschus manihot and Rhododendron arboreum. It’s protecting result on cultured PC12 cells against toxicity induced by hydrogen peroxide and tert-butyl hydroperoxide. Hyperin, isoquercitrin and quercetin isolated from ethyl acetate fraction of the root of A. chiisanensis showed lipopolysaccharide-induced nitrite production in rat peritoneal macrophages. As a crucial bioactive compound, hyperoside (hyperin) has been documented to possess antihyperglycemic, antiviral activity, antinociceptive, medicament, cardioprotective, hepatoprotective, and gastric-mucosal protecting result. The current study evaluates the ulcer healing potential of hyperin against absolute ethanol- (necrotizing agent), aspirin- (non-steroidal medicament drug) and histamine- (gastric secretion stimulator via H2 receptor) iatrogenic ulcers in rats. Hyperin (25 mg/kg and 50 mg/kg) was administered orally to the long fasted rats, one hour before the absolute ethanol/ aspirin/ histamine challenge. The ulcer index, gastroprotective potential, the status of the inhibitor enzymes enzyme (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) along with reduced glutathione (GSH), and lipid peroxidation were studied the models altogether. Pre-treatment with hyperin showed a dose-dependent decrease within the ulcer index altogether models. Hyperin at each dose, induced vital inhibitor activity by attenuating the ulcer elevated levels of MDA and fixed the ulcer-depleted levels of GSH, SOD, CAT, GPx, and GR. Hyperin possesses potent antiulcer activity, which can be attributed to an underlying antioxidant activity.