ANTIOXIDANT POTENTIAL OF PHLOROGLUCINOL; AN IN-VITRO APPROACHAbstract
Phloroglucinol is a polyphenol and a component of phlorotannins belonging to the Laminariaceae family. Phloroglucinol has many positive effects on health. The present study was aimed to evaluate the antioxidant potential of phloroglucinol by comparing it with Standard antioxidant viz, ascorbic acid. The antioxidant potential of phloroglucinol was estimated using free radicals such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion (O2–), nitric oxide (NO–), hydroxyl radical (OH), hydrogen peroxide (H2O2) by standard methods. The study shows that phloroglucinol is more powerful against DPPH and hydrogen peroxide radicals, when compared to other radicals. The IC50 value of Phloroglucinol is 42 ± 1.00 µg/ml for DPPH, 53.66 ± 1.52 µg/ml for Nitric oxide, 102 ± 2.00 µg/ml for Superoxide, 180 ± 3.60 µg/ml for Hydroxyl and 52.3 ± 1.52 µg/ml for Hydrogen peroxide radicals. In-vitro methods suggest that phloroglucinol is effective against DPPH and hydrogen peroxide radicals. Thus our study showed that the phloroglucinol exhibited antioxidant activity against all the free radicals and could be considered as a source of natural antioxidant.
I. Archana and K. Vijayalakshmi *
Department of Biochemistry, Bharathi Women’s College, Chennai, Tamil Nadu, India.
06 October, 2017
22 December, 2017
30 December, 2017
01 July, 2018