ANTIOXIDANT PROSPECTIVE AND SECONDARY METABOLITES IN ABUTILON INDICUM AT DIFFERENT ENVIRONMENTAbstract
To investigate the antioxidant property and secondary metabolites of Abutilon indicum Plant at various Habitats were compared with those of relevant callus cultures induced from every explant in in-vitro. The callus cultures were effectively initiated on Murashige and Skoog (MS) medium supplemented with 2, 4-D (1 mg/L) pooled with different concentrations (0.1–1.0 mg/L) of kinetin as plant growth regulators. The circulation of Flavonoids and phenolic compounds in the plant extracts were analyzed by using Aluminium (III) Chloride Colorimetric assay with standards. Flavonoids were found in all callus extracts in comparison with in their natural habitat plant parts at various Habitats. In this study, the antioxidant activities of the extracts were evaluated in vitro antioxidant-testing systems. The secondary metabolites of flavonoid and phenolic acid contents of Abutilon indicum were studied at dissimilar habitats and in-vitro callus culture extract. Among these studies hills and wet soil habit plants showed maximum secondary metabolites than the other habitats. The antioxidant potential was studied by dot blot assay. Between these results, we fulfilled that, the ecological stress factors such as dryness, temperature, salt and soil pH is a crucial factor for liberate secondary metabolites and antioxidant potent of Abutilon indicum.
Kandasamy Selvam*, Srinivasan Arunprakash , Thangasamy Selvankumar , Muthusamy Govarthanan and Arumugam Sengottaiyan
Department of Biotechnology, Mahendra Arts & Science College, Kalippatti-637 501, Tamilnadu, India
22 January, 2012
19 June, 2012
25 June, 2012
01 July 2012