ANTIOXIDANT STUDIES, ERYTHROCYTE MEMBRANE STABILIZATION AND THROMBOLYTIC POTENTIAL OF DIALLYL DISULPHIDEAbstract
Diallyl Disulphide is an essential component of garlic which has numerous medicinal applications. Thus it was evaluated for its free radical scavenging potential. The compound was assayed for its ability to stabilize the erythrocyte membrane by osmotic fragility test and heat induced hemolysis assay. The thrombolytic potential was evaluated by the ability of the compound to dissolve the clot formed. The antioxidant studies were assessed by DPPH radical scavenging assay, ferric reducing antioxidant potential, Superoxide radical scavenging potential and Nitric oxide radical scavenging potential. The total antioxidant capacity was also estimated and the values were expressed in terms of Ascorbic acid equivalents. The results show a poor free radical scavenging potential and antioxidant capacity. However it has shown a moderate percentage in dissolution of clot implicating in thrombolytic potential. The compound has also shown a higher erythrocyte membrane stabilization by reducing the percentage of hemolysis in heat induced hemolytic assay than Ascorbic Acid. The results suggest that Diallyl Disulphide is a poor antioxidant but a potential anti-hemolytic in In-vitro studies.
N. Suchetha Kumari *, T. Yogish Somayaji , R. Tejashvi , K. M. Damodara Gowda and C. Rao
Department of Biochemistry, K. S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India.
19 April, 2014
11 June, 2014
27 July, 2014
01 November, 2014