ANTIPLATELET AND ANTIHEMOLYTIC ACTIVITIES OF HYDROETHANOLIC EXTRACT OF ANNONA MURICATA LEAVESAbstract
This study designed to evaluate the antiplatelet and antihemolytic activity of hydroethanolic extract of Annona muricata leaves (HEEAM). The platelet aggregation assay was carried out using platelet-rich plasma, and the antihemolytic assay was done by hydrogen peroxide with different concentration of plant extract (100-500 μg/ml). HEEAM was found to possess more hydrogen donating ability when compared with the standard drug (aspirin). In addition, extract showed significant antihemolytic action (IC50 = 64.94 ± 0.01 µg/ml) against hydrogen peroxide (H2O2) induced hemolysis. It measures the degree of hemolysis when generated free radical damage. These were evident that the extracts exhibited strong antioxidant activity by protecting the red blood cells from hemolysis. Furthermore, the antiplatelet activity of HEEAM showed a maximum at 500 μg/ml concentration. It represents a promising treatment for thrombotic diseases. The percentage of hemolysis and platelet aggregation was calculated with standard aspirin. In conclusion, these observations suggest that HEEAM to posses a significant antiplatelet and antihemolytic potential.
N. Pichai and B. Durairaj *
Department of Biochemistry, PSG College of Arts and Science, Coimbatore, Tamil Nadu, India.
28 October 2019
25 April 2020
29 April 2020
01 October 2020