ANTITUBERCULAR POTENTIAL OF DENDROPHTHOE FALCATE (L.) AND TRIDAX PROCUMBENS (L.) PLANTS EXTRACTS AGAINST H37Rv STAIN OF MYCOBACTERIA TUBERCULOSISAbstract
The increasing incidence of MDR and XDR tuberculosis worldwide highlight the urgent need to search for newer anti-tuberculosis molecules. This research article presents a phytochemical study and antitubercular potential of methanol: water (MW), ethanol: water (EW) and dichloromethane: methanol (DM) of extracts of Dendrophthoe falcata (L.) and Tridax procumbens (L.) tested against of H37Rv Mycobacterium tuberculosis using microplate alamar blue assay (MABA) method. Preliminary phytochemicals studies and HPTLC fingerprint analysis revealed the presence of phytochemical like alkaloids, flavonoids, saponins, tannins, a phenolic group, glycosides, terpenoids with different Rf values. Fractions of active extracts analyzed by GC-MS shows a finding of probable phytoconstituents. Findings are useful to establishing standards for identification, purity, quality of the plant. The ethanol: water and methanol: water extracts of Tridax procumbens and D. falcata exhibited significant anti-tuberculosis activity with the MIC values of 0.8 μg/ml, 6.25 μg/ml compared to standard drug Pyrazinamide, Ciprofloxacin and Streptomycin with the MIC values of 3.125 μg/ml 6.25 μg/ml using MABA respectively against Mycobacterium tuberculosis (H37Rv strain) ATCC no.-27294. The presence of flavonoids, tannins, the phenolic group may contribute to the observed anti-tubercular activity. The study demonstrated that extract from T. procumbens and D. falcata could be evaluated further that might provide compounds for developing a new drug to control M. tuberculosis.
V. C. Bhagat * and M. S. Kondawar
Department of Pharmaceutical Chemistry, Rajgad Dnyanpeeth’s College of Pharmacy, Pune, Maharashtra, India.
11 May 2018
26 June 2018
04 July 2018
01 January 2019