APPLICATION OF SMARTPHONE AND DEVELOPMENT OF COLOUR KIT FOR THE ESTIMATION OF DOXYCYCLINE IN PHARMACEUTICAL DOSAGE FORMS AND ITS COMPARISON WITH A VALIDATED DIFFERENCE SPECTROSCOPIC METHOD BY UNCERTAINTYAbstract
In the proposed research paper, a simple, accurate, and precise colorimetric method has been developed for the estimation of doxycycline in bulk and pharmaceutical dosage forms. The proposed method utilizes a smartphone camera and an application instead of the UV spectrophotometer for the measurement of color intensity. This colorimetric method was found to be linear in the range of 10µg/ml to 50µg/ml concentration, with an r2 value of 0.9968. These results have been then compared with a highly selective difference spectroscopic method developed and validated by us using the principles of uncertainty. When we report the result of a measurement, it becomes obligatory that some quantitative indications of the quality of the results should also be given, which will aid in the assessment of the reliability of the results and, in turn, the method which was used to obtain those results. Without such an indication, the measurement results may not be compared, either amongst themselves or with the reference standard values. Hence, the uncertainty appraisal becomes essential to express the dispersion of the values that could be reasonably attributed to the results. The proposed difference spectroscopic method obeys Beer-Lambert’s Law in the concentration range of 10 – 30µg/ml with an r2 value of 0.999.
M. C. Trivedi and R. C. Mashru *
Faculty of Pharmacy, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India.
13 November 2019
26 February 2020
18 March 2020
01 November 2020