ASSESSMENT OF ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF SECONDARY METABOLITES FROM LEAVES AND FRUIT EXTRACT OF GYMNOSPORIA MONTANAAbstract
Objectives: Gymnosporia montana (family Celeastraceae), important shrub which is widely reported in Gujrat, India. In this study, our major objective is to screen secondary metabolites of methanolic leaves and fruit extract and determining its antioxidant and anti-inflammatory potential of this shrub. Materials and Methods: Leaves and fruit extract were subjected to methanol followed by phytochemical investigations and estimating the presence of secondary metabolites and also analysing its activity for antioxidant (DPPH and hydrogen peroxide scavenging methods) and anti-inflammatory potentiality were carried out. Results: Preliminary studies related to its phytochemical screening of leaves and fruit extract of Gymnosporia montana in the form of methanol extract which revealed its existence of flavonoids, alkaloids and terpenoids. In comparison with alkaloid and terpenoid extract, there is significant enhancement in antioxidant activity of flavonoid content in case of methanolic extract in a dose-dependent manner. Thereafter, itsanti-inflammatory activity test was carried out against Typhoid vaccine (25 µg/ml, 10 µl) with these extracts (flavonoids, terpenoids and alkaloids of methanol) using variable concentration and compared with Typhoid vaccine, used as standard. All these secondary metabolites extracts showed significant (especially seen in case of flavonoid from methanolic leaves and fruit extract) decline in antigen specific proliferationas compared to control, but there are no significant activities were observed in terpenoid and alkaloid. Conclusion: Overall, this study may indicate that methanol extract having secondary metabolite (flavonoid content) having antioxidant and anti-inflammatory properties.
Sandip Sutariya, Yashaswi Singh, A. B. Bajpai and Amit Gupta*
Department of Life Sciences (Biotechnology), Graphic Era (Deemed to be) University, Dehradun, Uttarakhand, India.
19 February 2022
23 March 2022
25 April 2022
01 October 2022