ASSESSMENT OF ANTIOXIDANT, CYTOTOXIC AND ANTIBACTERIAL ACTIVITIES OF DIFFERENT FRACTIONS OF CRUDE EXTRACT OF STEPHANIA JAPONICA STEMAbstract
The different fractions of crude methanolic extract of stem of Stephania japonica (Thunb.) Miers was evaluated for antioxidant, cytotoxic and antibacterial activities. The various fractions of S. japonica Stem i.e. Ethyl Acetate (EAJS), CHCl3 (CFJS), CCl4 (CTJS) and Petroleum ether (PEJS) were subjected to free radical scavenging activity. In this investigation, CFJS showed the most significant free radical scavenging activity with IC50 value of 119.0µg/ml for S. japonica stem. Cytotoxic activity was investigated by brine shrimp (Artemia salina) lethality assay. The LC50 value of sample CTJS (Carbon Tetrachloride fraction of S. japonica Stem), was 3.0µg/ml is highly most significant. Antibacterial activity was tested by disk diffusion method. The Carbon Tetrachloride soluble fraction showed good antibacterial activity against different species of bacteria at different doses. The Ethyl Acetate soluble fraction shows good activity only against E. coli at different doses.
Bishwajit Bokshi , S.M. Abdur Rahman*, Samir Kumar Sadhu , Ashif Muhammad and Hemayet Hossain
Professor, Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Bangladesh
25 December, 2012
13 March, 2013
28 March, 2013
01 April, 2013