ASSOCIATION OF TLR2 AND IL-8 POLYMORPHISMS AND THEIR EXPRESSION IN GUILLAIN-BARRÉ SYNDROMEAbstract
Guillain-Barré syndrome (GBS) is an acute inflammatory, autoimmune disorder of peripheral nervous system. Association of TLR2 and IL-8 polymorphism with higher expression level has already been studied in many inflammatory and autoimmune diseases. However, the possible role of TLR2 and IL-8 polymorphism in GBS remains unknown. Therefore, the current study investigated the TLR-2 (Arg677Trp & Arg753Gln) and IL-8 (-251A/T) polymorphisms in 105 GBS patients and 100 healthy controls using polymerase chain reaction-restriction fragment length polymorphism analysis. Further, the expression of TLR-2 and IL-8 gene was determined by western blotting and enzyme-linked immunosorbent assay respectively. TLR2 (Arg677Trp & Arg753Gln) heterozygous genotypes were strongly associated with increased risk of GBS. TLR2 and IL-8 genes showed significantly higher expression in GBS when compared with healthy controls. In conclusion, TLR2 polymorphisms showed significant association with GBS, and their enhanced expressions and increased level of IL-8 have possible role in GBS development. TLR2 polymorphisms could be a genetic marker to GBS susceptibility.
N. K. Kharwar, K. N. Prasad *, M. Rai, V. K. Paliwal and D. R. Modi
Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow (U.P.), India
12 April, 2016
15 June, 2016
29 June, 2016
01 September 2016