BIO ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF TEMOZOLOMIDE IN RAT PLASMA USING RP-HPLC METHODAbstract
A simple high-performance liquid chromatographic method was developed and validated for the determination of Temozolomide in rat plasma using paracetamol as internal standard. The chromatographic separation was carried out with an isocratic elution using mobile phase consisting of 0.5% glacial acetic acid and methanol. Plasma samples were run at a flow-rate of 1 ml/min using a kromasil C18(4.6×250mm) column and ultraviolet detection at a wavelength of 265nm. The retention times of Temozolomide and paracetamol were found to be 6.92 and 11.2 minutes respectively. The linearity of the method was achieved over the concentration range of 10-60µg/ml demonstrated good linearity with correlation coefficient 0.999. The method was validated for accuracy, precision, linearity, recovery in compliance to international regulatory guidelines
Swathimutyam Pallerla* and Bala Prabhakar
Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management, Vile Parle, Mumbai, Maharashtra, India.
23 September, 2015
16 November, 2015
05 January, 2016
01 March, 2016