BIO-ANALYTICAL STUDIES ON THE PROCESS OF DETOXIFICATION AND SAFETY EVALUATION OF ACONITUM LACINIATUM AND ABRUS PRECATORIUS FOR USE IN AYURVEDIC PREPARATIONS

Objective: The aim of the study was to have bio-analytical approach for detoxification process of two poisonous plant materials viz. Aconitum laciniatum (root) and Abrus precatorius (seed) which are used in Ayurvedic preparations.

Materials and Methods: For both the species proximate analysis was carried out. For the same plants HPTLC fingerprinting was compared , before and after detoxification process, using triphala quath. Infra red spectral studies for Abrus precatorius species (red and white) were compared, with respect to detoxification process. Protein fingerprinting was carried out for various Aconitum species available in the market. In support of the results obtained from the above methods for detoxification, safety evaluation , post single  dose administration,  C1- post single dose administration]was done using albino mice as the study model for Aconitum laciniatum (root) and Abrus precatorius (seeds- red and white).

Outcome Measures: Comparison of the results for the crude poisonous herbal material with the material obtained after detoxification in triphala quath.

Results: HPTLC fingerprinting, Infra red spectral studies, safety evaluation study (animal toxicity) showed that process of detoxification for the above mentioned plant materials using triphala quath is effective and less time consuming.

Conclusions: The study highlights that the commonly used poisonous crude herbal materials viz. Aconitum laciniatum (root) and Abrus precatorius (seeds – red and white) can be safely used in Ayurvedic preparations, after detoxification using triphala quath.