BIOLOGICAL INVESTIGATIONS OF THE LEAF EXTRACT OF SPONDIAS PINNATAAbstract
The present study was done to evaluate in-vitro membrane stabilizing activity with human RBC, in-vivo neuropharmacological activity including open field and swimming test, gastrointestinal motility and acute toxicity of different leaf extracts of Spondias pinnata in Swiss albino mice following oral administration. In-vivo GI motility test was done by charcoal induced anti motility test, acute toxicity study was done by investigating mortality/morbidity status of test animal and in-vitro membrane stabilizing activity was done with human RBC in both hypotonic and heat induced method. In case of in-vitro membrane stabilizing activity for hypotonic solution induced haemolysis, the ethanol extract inhibited 76.92% haemolysis of RBCs as compared to 85.42% produced by acetyl salicylic acid and during heat induced condition different organic soluble materials of S. pinnata demonstrated 76.68%, 77.19% and 74.83% inhibition of haemolysis of RBCs respectively whereas ASA inhibited 78.04%. In-vivo neuropharmacological activity showed significant (p<0.05) and (p<0.001) results when methanol 200 mg/kg, ethanol 200 mg/kg, chloroform 100 & 200 mg/kg extracts were administered to evaluate the rate of movement with time in dose dependent manner when compared with corresponding value of control group. In case of forced swimming test methanol 200 mg/kg, ethanol 200 mg/kg, chloroform 100 & 200 mg/kg extracts showed significant (p<0.001) and (p<0.05) results corresponding value of control group. Both the dosage of methanol and ethanol extracts of 100 mg/kg and 200 mg/kg showed significant (p<0.05) and (p<0.001) results in gastrointestinal motility comparing with standard. None of the extracts showed any in-vivo acute toxicity effect on mice.
S. Kamal, R. Akhter, N. Akter Tithi, Md. A. Wadud, S. N. Narjish, Md. Shahriar and M. A. Bhuiyan*
Phytochemistry Research Laboratory, Department of Pharmacy, University of Asia Pacific, Dhaka, Bangladesh
18 December, 2014
18 February, 2015
26 April, 2015
01 August, 2015