BIOLOGICAL SCREENING AND PHYTOCHEMICAL INVESTIGATION OF MERREMIA EMARGINATA LEAVESAbstract
The dried leaves of Merremia emarginata Burm. F were screened for antitubercular, cytotoxic, and anti-inflammatory activities. The antimycobacterial activity of compounds was assessed against Myco-bacterium tuberculosis using microplate Alamar Blue assay (MABA), and the cytotoxic activity was studied on Hela (Human cervical carcinoma), MCF-7 (Human breast carcinoma) cell lines. A prenylflavonoid, 8-prenylnaringenin was isolated on silica thin layer chromatography, and the radical scavenging efficiency of the isolated compound was evaluated by DPPH radical assay. The results demonstrated that the isolated compound was found to be greater than that of BHT from lower (0.25 mM) to moderate (2 mM) concentrations and comparable to BHT at higher concentrations, up to 4 mM. The flavonoid was also found to exhibit good antifungal activity when tested against Candida albicans (ATCC 2091) and Aspergillus niger (ATCC 16404).
C. Devadasu *, R. Y. Prasad, S. P. Babu and S. Gananadhamu
School of Pharmacy, Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh India.
14 February 2019
25 August 2020
12 December 2020
01 January 2021