BRINE SHRIM LETHALITY BIOASSAY OF METHANOLIC CRUDE EXTRACTS OF TERMINALIA ARJUNAAbstract
The aim of this work is to determine the cytotoxic activities of Terminalia arjuna (Family: Combretaceae) by brine shrimp lethality bioassay. The leaves of Terminalia arjuna were extracted with methanol and the methanolic extract was fractionated into three fractions by using organic solvents like n-hexane, ethyl acetate and chloroform. The n-hexane, ethyl acetate and chloroform soluble fractions of methanolic crude extract were screened for antitumor properties using brine shrimp lethality bioassay. A reputed cytotoxic agent vincristine sulphate was used as a positive control. From the results of the brine shrimp lethality bioassay it could be well predicted that n- hexane, ethyl acetate and chloroform soluble fractions of Terminalia arjuna leaves possess cytotoxic principles (LC50 1.29 µg/mL, 1.5 µg/mL and 1.23 µg/mL respectively) comparison with positive control, vincristine sulphate (LC50 0.563 µg/mL).
Md. Anaet Hossen Maruf and Mohammad Musarraf Hussain *
Assistant Professor, Department of Pharmacy, Jagannath University, Dhaka-1100 Bangladesh
02 April, 2012
21 October, 2012
29 October, 2012