BRINE SHRIM LETHALITY BIOASSAY OF METHANOLIC CRUDE EXTRACTS OF TERMINALIA ARJUNA
AbstractThe aim of this work is to determine the cytotoxic activities of Terminalia arjuna (Family: Combretaceae) by brine shrimp lethality bioassay. The leaves of Terminalia arjuna were extracted with methanol and the methanolic extract was fractionated into three fractions by using organic solvents like n-hexane, ethyl acetate and chloroform. The n-hexane, ethyl acetate and chloroform soluble fractions of methanolic crude extract were screened for antitumor properties using brine shrimp lethality bioassay. A reputed cytotoxic agent vincristine sulphate was used as a positive control. From the results of the brine shrimp lethality bioassay it could be well predicted that n- hexane, ethyl acetate and chloroform soluble fractions of Terminalia arjuna leaves possess cytotoxic principles (LC50 1.29 µg/mL, 1.5 µg/mL and 1.23 µg/mL respectively) comparison with positive control, vincristine sulphate (LC50 0.563 µg/mL).
Article Information
35
4297-4300
619KB
986
English
Ijpsr
Md. Anaet Hossen Maruf and Mohammad Musarraf Hussain *
Assistant Professor, Department of Pharmacy, Jagannath University, Dhaka-1100 Bangladesh
m.musarraf.hussain@gmail.com
02 April, 2012
21 October, 2012
29 October, 2012
http://dx.doi.org/10.13040/IJPSR.0975-8232.3(11).4297-00
01 November,2012
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