CAFFEINE AND P-ANISALDEHYDE FROM THE FRUITS OF ENTEROLOBIUM SAMAN PRAINAbstract
Fruits of the plant were air dried for several days and cut into small pieces, which were subjected to oven dried for 24 hours at 45-50°C to effect further chopping. Cold methanol extract of fruits were subjected to acid-base treatment to separate the alkaloids. The treated extract was subjected to column chromatography to isolate the pure compound. The xanthine alkaloid, 1, 3, 7-trimethyl-1H-purine-2, 6 (3H, 7H)-dione, caffeine (compound 1) and also p-anisaldehyde (compound 2) were isolated from the column fraction of this ‘alkaloid containing residue’ by elution with ethyl acetate/10% methanol and ethyl acetate/50-100% methanol, respectively. The isolated pure compounds were identified by extensive spectral (1H-NMR, 13C-NMR, DEPT, HMBC, HSQC, Mass) data analysis. Greshoff isolated an alkaloid, pithecolobin from the bark, which is saponin like in its action. Wehmer stated that the seeds also contain pithecolobin. In the Philippines, a decoction of the inner bark (fresh cambium) and the fresh leaves of this tree is used in diarrhea. All these above mentioned study lead us to work on it.
Mohammad Kaisarul Islam*, Md. Hossain Sohrab and Abdul Jabbar
Department of Pharmaceutical Chemistry, University of Dhaka, Dhaka-1000, Bangladesh
02 September, 2011
21 December, 2011
29 December, 2011