CHARACTERISATION OF FLAVONOID GLYCOSIDES IN PHARMACOPOEIAL PREPARATION OF CISSUS VERTICILLATA (L) NICOLSON & C. E. JARVIS) USING HPLC-DAD AND HPLC-MS

According to Brazilian regulation, the registration of phytomedicines must inform the characterisation of, at least, one of their constituent. In order to contribute to this goal, a tincture of Cissus verticillata (L.) Nicolson & C.E. Jarvis prepared with ethanol 96^oGL was investigated. This plant species is widely used due its hypoglycaemic properties, to control diabetes, and empirically to treat the consequence of cerebral vascular accident. Thus, this work reports the evaluation of the flavonoid content in the tincture and the characterisation of flavonoid glycosides, because flavonoids can be part of the active constituents of this herbal drug, and also because these substances can be used as markers. This article both confirms the presence of several flavonoids previously detected in this herbal drug and reports the presence of others not previously described and are characterised in this species for the first time. The flavonoid content in the tincture, determined as Rutin using UV spectroscopy, is 3.6g% of tincture. A method based on reversed phase (RP) HPLC analysis was developed and allowed good separation of the flavonoids found in C. verticillata. Aglycones and both O– and C-derivatives of the flavonoids are easily and also adequately separated.