CHARACTERIZATION AND HPLC ANALYSIS OF MANNA FROM SOME COTONEASTER SPECIESAbstract
Cotoneaster is a plant from Rosaceae family and produces a sugary secretion (manna). Manna is the most important constituent of species of this plant. Manna consists of different types of carbohydrates, and other phytochemicals. Sugar characterization, including reducing sugar was assayed by cupric/sodium carbonate spectrophotometric method. In addition, the composition of individual sugars was also determined using high-performance liquid chromatography (HPLC) coupled with a refraction index (RI) detector, with mannitol being the most abundant sugar. Also, amount of essential microelements was determined using flame atomic absorption spectrometry. Finally, total flavonoid content in all Cotoneaster species was determined using the aluminum chloride method. According to HPLC-RI data, C. nummularioides manna has a high percentage of mannitol (88.69±0.05) and low amount of sucrose (5.37±0.01). Total reducing sugar of the C. discolor is the lowest and in C. nummularius is the highest among all mannas. The study shows differences in sugar profile, essential microelements, and total flavonoid content. Mannas from Cotoneaster species have different carbohydrate profiles and other phytochemicals and have different pharmacologic effects for these compounds.
M. Fakhri, A. Davoodi, M. Parviz, Z. S. Ghadi, S. N. Mousavinasab, R. Farhadi, M. Azadbakht and M. Azadbakht*
Department of Pharmacognosy, Bu-Ali Hospital, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
18 April, 2017
21 June, 2017
25 July, 2017
01 December, 2017